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Objective To evaluate the diagnostic value of lymph node fine-needle aspiration (FNA)Epstein-Barr virus (EBV)-DNA concentration detection in nasopharyngeal carcinoma (NPC) cervical lymph node metastasis.Methods From August to December 2016,36 cases of NPC and 9 cases of other tumors (not correlated with EBV infection) were enrolled in this study at the Sun Yat-sen University Cancer Center.All patients received magnetic resonance images (MRI),plasma and cervical lymph node FNA EBV-DNA detection.Results The median concentration of EBV-DNA in FNA fluid (1.39 × 105 copies/ml) in cervical lymph node metastasis was significantly higher than that in plasma (2.00 × 103 copies/ml),with a significant difference (x2 =16.723,P =0.004).The diagnosis sensitivity,specificity,accuracy of the lymph node FNA fluid of EBV-DNA were 86.2％ (25/29),71.4％ (10/14) and 81.4％ (35/43) respectively,which were better than those of MRI [72.4％ (21/29),50.0％ (7/14) and 65.1％ (28/43) respectively] and plasma EBV-DNA [55.2％ (16/29),71.4％ (10/14) and 60.5％ (26/43) respectively].The area under the curve (AUC) of level Ⅰ b cervical lymph node metastasis was calculated,and FNA fluid EBV-DNA (AUC =0.688)was better than MRI (AUC =0.583),with a significant difference (Z =2.476,P =0.008).The EBV-DNA concentration in FNA fluid in cervical lymph node metastasis of patients with other tumors (no correlated with EBV infection) was 0 copy/ml.Conclusion FNA fluid EBV-DNA may improve the diagnostic sensitivity of cervical lymph node metastasis in nasopharyngeal carcinoma,and help to explore the clinical target volume neck nodes at level Ⅰ b cervical lymph node in radiotherapy.
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Objective To investigate the clinical features, effective treatment, survival and prognostic factors of second primary tongue squamous cell carcinoma (SPTSCC) after nasopharyngeal carcinoma (NPC) radiotherapy. Methods The clinical data of 35 cases with SPTSCC after NPC radiotherapy were analyzed retrospectively. Kaplan-Meier method, Log-Rank test and COX proportional hazard mode was performed for statistical analysis. Results 3-year and 5-year overall survival rates were 55 % and 47 %, respectively, lymph node metastasis rate was 5.71 %. Univariate analysis indicated that gender (χ2 = 8.89, P = 0.00), T classification (χ2= 5.58, P= 0.02), clinical stage (χ2 = 8.51, P= 0.04) and treatment methods (χ2 = 29.37, P = 0.00) were important factors of prognosis. Multivariate analysis showed that treatment methods (P = 0.00) and T classification (P = 0.03) were independent prognostic factors. Operative treatment group had better prognosis than the non-operative treatment group, the difference was statistically significant (P <0.05), male patients in the risk of SPTSCC was higher than the female patients, and the incidence of SPTSCC was increased along with extension of the time after NPC radiotherapy. Conclusion The rate of the lymph node metastasis is lower for SPTSCC after NPC radiotherapy and treatment patterns and T stage are independent prognostic factors. Long-term follow-up after NPC radiotherapy is necessary to the early diagnosis of SPTSCC, so that to give surgery or combined therapy with surgery in order to achieve a good effect.
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OBJECTIVE@#To investigate the correlation between intratumor microvessel density(IMVD) and lymph node micrometastases.@*METHOD@#IMVD and lymph node micrometastases were stained with CD105 and CK19 antibody by immunohistochemical method of SABC respectively.@*RESULT@#IMVD marked with CD105 antibody correlated with T stage and lymph node micrometastases (P < 0.01).@*CONCLUSION@#The result show that IMVD marked with CD105 antibody highly correlated with lymph node micrometastases. It could be a index to evaluate the prognosis of patients with pN0 supraglottic laryngeal squamous cell carcinoma.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Pathology , Laryngeal Neoplasms , Pathology , Lymph Nodes , Pathology , Lymphatic Metastasis , Microvessels , Neoplasm StagingABSTRACT
Objective To assess the status of mental health of nursing students of secondary technical school and provide targeted guidance for mental health education. Methods 642 nursing students of secondary technical school were assessed using Mental Health Diagnostic Test Scale (MHT Scale). Results There were 0.78% of nursing students with serious psychological problems (scores≥65), 4.52% of nursing students with less serious psychological problems (scores < 65 and≥ 56). Students in different grades had significant difference in learning anxiety, allergic tendency, physical symptoms anxiety. Conclusions Nursing students of secondary technical school were in good mental health in general, but there were many students had learning anxiety, allergic tendencies and physical symptoms anxiety, which were different among various grades. Secondary nursing schools should further strengthen the mental health education, and seek close collaboration with parents,provide appropriate intervention measures to the nursing students with psychological barriers, in order to enhance the overall mental health of nursing students.
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Objective:To investigate the correlation between intratumor microvessel density (IMVD) and lymph node micrometastases. Method: IMVD and lymph node micrometastases were stained with CD105 and CK19 antibody by immunohistochemical method of SABC respectively. Result:IMVD marked with CD105 antibody corre-lated with T stage and lymph node micrometastases(P<0.01). Conclusion: The result show that IMVD marked with CD105 antibody highly correlated with lymph node micrometastases. It could be a index to evaluate the prog-nosis of patients with pN_0 supraglottic laryngeal squamous cell carcinoma.
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Objective To explore the relationship between the expression of P53 protein and the radiosensitivity in patients with advanced maxillary squamous cell carcinoma.Methods An immunohistochomical method wag used to detect the expression of P53 protein in patients with advanced maxillary squamous cell carcinoma.The follow up time was 2 years.The local recurrence of the patients having been treated with radical surgery and affiliated radiotheraphy were analyzed.Results The overexpression of P53 protein in 26 cages was 65.4% (17/26).In the P53 overexpression group,the local recurrence after systiem therapy wag occurred in one case within 6 months,6 cages between 7～12 months.3 cages between 13～18 months and 2 cases between 19～24 months.In the P53 low expression group,there were no recurrence within 6 and 12 months and one case ocurred within 18 months.5 cages between 19～24 months.The difference of recurrence within 18 months after system therapy between the expression of P53 Wag statistically significant(P＜0.05),but it Wag not significant for those within 24 months(P＞0.05).Conclusions The expression of P53 protein Wag correlated with the radiosensitivity in patient with advaneed maxillary squamous cell carciaoma,especially for the resid.rod cells in mitosis phase.The affiliated radiotherapy after radical surgery Wag limited effect.
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Objective: To explore the IL\|5 values in the tissue and secretion of nasal polyp. Methods: The concentrations of IL\|5, either in tissue homogenate or in nasal secretion, were measured by ELISA. The values of total protein in tissue homogenate were measured by Bradford colorimetry. In this study, 31 cases with nasal polyp, 8 patients with perennial allergic rhinitis (PAR), 11 cases with chronic sinusitis (CS) and 6 control cases were chosen. Results: ① In tissue homogenate, the IL\|5 concentrations of nasal polyp were higher than that in CS or control group (P＜0.001). ② In nasal secretion, IL\|5 values of both nasal polyp and PAR group were significantly higher than that in CS or control group (P＜0.05). ③ When all cases or only patients with nasal polyp were taken as the subjects, the Pearson correlation coefficient were 0.70, 0.66 respectively. Conclusions: IL\|5 values, both in tissue homogenate and nasal secretion, are significantly higher, which suggested that there were a close relationship between IL\|5 and nasal polyp, and IL\|5 play a key role in the formation of nasal polyp.
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Objective:To explore the expression of IL-5 and its role in the formation and development ofnasal polyp. Method: 31 patients with nasal polyp, 11 patients with chronic sinusitis (CS) and 6 control cases werechosen . Their IL-5 concentration in tissue homogenate were measured by ELISA. All patients with chronic si-nusitis,control cases and 15 patients with nasal polyp were chosen to be counted eosinophils in their HE slice.Result :①The values of IL-5(pg/ml) in nasal polyp,CS and control group separately were .. 23.44± 6.68,16.41±3.09,12.86±4.17. IL-5 concentration in nasal polyp group was higher than that in the other two groups( P ＜0. 001). ②The numbers of eosinophils in nasal polyp ,CS and control group were 7.42±2.33,1.30± 0. 59,1.07±0.70 separately,the numbers of eosinophils in nasal polyp group were higher than that in CR or control group(P ＜ 0.05). Conclusion: Nasal polyp is a disease characterized by eosinophilia, and IL-5 which activateseosinophil plays part role in the formation of nasal polyp.
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Purpose:To investigate the clinical applicati on and curative effect of salvage treatment for primary recurrence after definitiv e radiotherapy in patients with laryngeal cancer. Methods:52 patients with laryngeal cancer receiving definitive radiotherapy in the Cancer Center of Sun Yat-sen University between 1990 and 19 95 were reviewed retrospectively. Among them, 17 patients presented with primary recurrence. Salvage treatment after radiotherapy failure included palliative ch emotherapy (5 cases) and surgical salvage (12 cases). 6 patients were salvaged b y partial laryngectomy and 6 patients by total laryngectomy. SPSS 10.0 software was used to analyze the effects, complications and the differences between diffe rent salvage treatments. Results:The overall 3- and 5-year cumulative survival rates a fter salvage treatment for primary recurrence in patients with laryngeal cancer receiving definitive radiotherapy were 56.3% and 37.5% respectively. The patient s receiving palliative chemotherapy after radiotherapy failure survived between 8 and 26 months. The 3- and 5-year cumulative survival rate in patients receiv ing surgical salvage were 75% and 50% respectively. There was significant differ ence between them analyzed by Kaplan-Meier (Log Rank=8.14, P=0.004 3). More over, the 5-year survival rates in patients salvaged by partial laryngectomy an d total laryngectomy were all 50% and there was no statistical difference betwee n them (Log Rank=0.08, P=0.7782). 5 out of 12 (41.7%) patients salvaged by s urgery presented complication after surgery, mainly infection after surgery (25 %) and pharyngocutaneous fistula (25%). Conclusions:Surgical salvage for primary recurrence in patients with laryngeal cancer receiving definitive radiotherapy could be effective.Part ial laryngectomy could be used for early primary lesion (T 1 and T 2) in patie nts with primary recurrence. The complications after salvage surgery were mainly were infection and pharyngocutaneous fistula.